LNX1 Polyklonaler Antikörper

LNX1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13124-1-AP

Synonyme

ligand of numb protein X 1, LNX, LNX1, MPDZ, Numb binding protein 1, PDZRN2

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Galerie der Validierungsdaten

• 

  WB analysis of mouse testis using 13124-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13124-1-AP (LNX1 antibody) at dilution of 1:70 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13124-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13124-1-AP (LNX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

13124-1-AP bindet in WB, ELISA LNX1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LNX1 fusion protein Ag3823
• Vollständiger Name: ligand of numb-protein X 1
• Berechnetes Molekulargewicht: 728 aa, 81 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC022983
• Gene symbol: LNX1
• Gene ID (NCBI): 84708
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LNX1 (Ligand of Numb, protein X 1) was originally identified as a binding partner of the cell-fate determinant Numb and was reported to be involved in ubiquitylation and degradation of Numb. LNX1 contains four PDZ domains and may function as a scaffold for a multi-protein complex. LNX1 has two splicing variants, LNX1p70 and LNX1p80. LNX1p70 has the N-terminal Numb binding region and four PDZ domains, whereas LNX1p80 has an additional RING-finger domain at the N-terminus followed by a Numb-binding region and four PDZ domains. This antibody can detect both LNX1p70 and LNX1p80, around 70-80 kDa and 80-100 kDa, respectively.