LOH12CR1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

LOH12CR1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 17169-1-AP

Synonyme

BLOC-1-related complex subunit 5, BORCS5, LOH1CR12, Loss of heterozygosity 12 chromosomal region 1, Myristoylated lysosomal protein

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  WB analysis using 17169-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17169-1-AP (LOH12CR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 17169-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17169-1-AP (LOH12CR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 17169-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17169-1-AP (LOH12CR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17169-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17169-1-AP (LOH12CR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17169-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17169-1-AP (LOH12CR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 17169-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 17169-1-AP (LOH12CR1 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below

Produktinformation

17169-1-AP bindet in WB, IHC, IF/ICC, ELISA LOH12CR1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LOH12CR1 fusion protein Ag10695
• Vollständiger Name: loss of heterozygosity, 12, chromosomal region 1
• Berechnetes Molekulargewicht: 196 aa, 22 kDa
• Beobachtetes Molekulargewicht: 17-22 kDa
• GenBank-Zugangsnummer: BC013668
• Gene symbol: LOH12CR1
• Gene ID (NCBI): 118426
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LOH12CR1, also named as BORCS5, is part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. It may indirectly play a role in cell spreading and motility. LOH12CR1 has two isoforms with MW 17 kDa and 22 kDa.

Publikationen

Species	Application	Title
mouse,human	WB	Dev Cell; BORC, a Multisubunit Complex that Regulates Lysosome Positioning.; Authors - Jing Pu
human	WB	Autophagy; HIV-1 Vpr protein impairs lysosome clearance causing SNCA/alpha-synuclein accumulation in neurons.; Authors - Maryline Santerre
		Cell Rep; Consecutive functions of small GTPases guide HOPS-mediated tethering of late endosomes and lysosomes; Authors - Ariane Schleinitz
mouse	WB	Cell Rep; Synaptic Vesicle Precursors and Lysosomes Are Transported by Different Mechanisms in the Axon of Mammalian Neurons.; Authors - Raffaella De Pace ; KO Validated
human	WB	Cell Genom; Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders; Authors - Greta Pintacuda
		Nat Neurosci; Messenger RNA transport on lysosomal vesicles maintains axonal mitochondrial homeostasis and prevents axonal degeneration; Authors - Raffaella De Pace