LONP1 Monoklonaler Antikörper

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LONP1 Monoklonal Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C6C12

Kat-Nr. : 66043-1-PBS

Synonyme

Mitochondrial ATP-dependent protease Lon, LONP 1, LONP, LON, EC:3.4.21.53

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  WB analysis using 66043-1-Ig (same clone as 66043-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66043-1-Ig (same clone as 66043-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66043-1-Ig (same clone as 66043-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 66043-1-Ig (same clone as 66043-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 66043-1-Ig (same clone as 66043-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66043-1-Ig (same clone as 66043-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66043-1-Ig (same clone as 66043-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 66043-1-Ig (same clone as 66043-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66043-1-Ig (same clone as 66043-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66043-1-Ig (same clone as 66043-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66043-1-Ig (same clone as 66043-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66043-1-Ig (same clone as 66043-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66043-1-Ig (same clone as 66043-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66043-1-Ig (same clone as 66043-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66043-1-Ig (same clone as 66043-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66043-1-Ig (same clone as 66043-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

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  IF Staining of C6 using 66043-1-Ig (same clone as 66043-1-PBS)

  Immunofluorescent analysis of C6 cells using 66043-1-Ig (LONP1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

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  IF Staining of C6 using 66043-1-Ig (same clone as 66043-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using LONP1 antibody (66043-1-Ig, Clone: 1C6C12 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66043-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50672-1

  Cytometric bead array standard curve of MP50672-1, LONP1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66043-1-PBS. Detection antibody: 66043-2-PBS. Standard:Ag7306. Range: 1.563-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66043-1-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA LONP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LONP1 fusion protein Ag7306
• Vollständiger Name: lon peptidase 1, mitochondrial
• Berechnetes Molekulargewicht: 106 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC000235
• Gene symbol: LONP1
• Gene ID (NCBI): 9361
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LONP1(Lon protease homolog, mitochondrial) is also named as LONP, LONHS, HLON, LON, PRSS15, PIM1, MGC1498 and belongs to the peptidase S16 family. It seems to play a major role in the elimination of oxidatively modified proteins in the mitochondrial matrix(PMID:18021745). LONP1, also a nuclearly encoded and mitochondrially located stress-responsive protease, is involved in heme-mediated ALAS-1 turnover(PMID:21659532). It recognizes specific surface determinants or folds, initiates proteolysis at solvent-accessible sites, and generates unfolded polypeptides that are then processively degraded(PMID:15870080).