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LONP1 Rekombinanter Antikörper

LONP1 Rekombinant Antikörper für IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240460E5

Kat-Nr. : 83552-3-PBS

Synonyme

240460E5, EC:3.4.21.53, LON, LONP, LONP 1

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LONP1 Recombinant antibody (83552-3-RR)

    IF Staining of HepG2 using 83552-3-RR (same clone as 83552-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LONP1 antibody (83552-3-RR, Clone: 240460E5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83552-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using LONP1 Recombinant antibody (83552-3-RR)

    IF Staining of HepG2 using 83552-3-RR (same clone as 83552-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LONP1 antibody (83552-3-RR, Clone: 240460E5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83552-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using LONP1 Recombinant antibody (83552-3-RR)

    FC experiment of HeLa using 83552-3-RR (same clone as 83552-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug LONP1 Recombinant antibody (83552-3-RR, Clone:240460E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83552-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00531-3

    Cytometric bead array standard curve of MP00531-3

    Cytometric bead array standard curve of MP00531-3, LONP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83552-3-PBS. Detection antibody: 83552-1-PBS. Standard: Ag7196. Range: 0.938-60 ng/mL.

  • Affinity and Kinetic Characterization of 83552-3-RR

    Affinity and Kinetic Characterization of 83552-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83552-3-RR against Human LONP1 were performed. The affinity constant is 1.38 nM.

Geprüfte Anwendungen

Produktinformation

83552-3-PBS bindet in IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA LONP1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen LONP1 fusion protein Ag7196
Vollständiger Name lon peptidase 1, mitochondrial
Berechnetes Molekulargewicht 106 kDa
GenBank-ZugangsnummerBC000235
Gene symbol LONP1
Gene ID (NCBI) 9361
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.