LOX Polyklonaler Antikörper

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LOX Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17958-1-PBS

Synonyme

Protein-lysine 6-oxidase, short form, Protein-lysine 6-oxidase, long form, Protein-lysine 6-oxidase, lysyl oxidase, EC:1.4.3.13

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  WB analysis of Jurkat using 17958-1-AP (same clone as 17958-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 17958-1-AP (same clone as 17958-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

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  WB analysis of MKN-45 using 17958-1-AP (same clone as 17958-1-PBS)

  MKN-45 cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

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  WB analysis of SGC-7901 using 17958-1-AP (same clone as 17958-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 17958-1-AP (same clone as 17958-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 17958-1-AP (same clone as 17958-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17958-1-AP (LOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse skeletal muscle using 17958-1-AP (same clone as 17958-1-PBS)

  IP result of anti-LOX (IP:17958-1-AP, 3ug; Detection:17958-1-AP 1:500) with mouse skeletal muscle tissue lysate 2000ug. This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 17958-1-AP (same clone as 17958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17958-1-AP (LOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 17958-1-AP (same clone as 17958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 17958-1-AP (LOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 17958-1-AP (same clone as 17958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 17958-1-AP (LOX antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 17958-1-AP (same clone as 17958-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 17958-1-AP (LOX antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17958-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17958-1-PBS bindet in WB, IHC, IP, Indirect ELISA LOX und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LOX fusion protein Ag12479
• Vollständiger Name: lysyl oxidase
• Berechnetes Molekulargewicht: 417 aa, 47 kDa
• Beobachtetes Molekulargewicht: 32 kDa, 45-47 kDa
• GenBank-Zugangsnummer: BC074872
• Gene symbol: LOX
• Gene ID (NCBI): 4015
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LOX is a member of a multigene family (LOX, LOXL, LOXL2, LOXL3, and LOXL4). Lysyl oxidase (LOX), a copper-dependent enzyme, plays a critical role in the formation and repair of the extracellularmatrix (ECM) by catalyzing the crosslinking of elastin and collagen. LOX expression appears to be upregulated in malignancies and has recently been suggested to play a role in regulating tumor metastasis. It is synthesized as a 50 kDa proenzyme, secreted, and processed into an 32 kDa mature, active enzyme and an 18-kDa propeptide.