LPCAT1 Monoklonaler Antikörper

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LPCAT1 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

8B6E9

Kat-Nr. : 66044-1-PBS

Synonyme

Acetyl-CoA:lyso-PAF acetyltransferase, 8B6E9, 1-alkylglycerophosphocholine O-acetyltransferase, 1-alkenylglycerophosphocholine O-acyltransferase, 1-acylglycerophosphocholine O-acyltransferase

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  WB analysis using 66044-1-Ig (same clone as 66044-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66044-1-Ig (same clone as 66044-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  WB analysis of ROS1728 using 66044-1-Ig (same clone as 66044-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-12 using 66044-1-Ig (same clone as 66044-1-PBS)

  PC-12, C6, Raw264.7, NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66044-1-Ig (same clone as 66044-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66044-1-Ig (LPCAT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66044-1-Ig (same clone as 66044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66044-1-Ig (same clone as 66044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66044-1-Ig (same clone as 66044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66044-1-Ig (same clone as 66044-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66044-1-Ig (LPCAT1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse lung using 66044-1-Ig (same clone as 66044-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using LPCAT1 antibody (66044-1-Ig, Clone: 8B6E9 ) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), SFTPC antibody (10774-1-AP, green). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66044-1-Ig (same clone as 66044-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LPCAT1 antibody (66044-1-Ig, Clone: 8B6E9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66044-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50776-3

  Cytometric bead array standard curve of MP50776-3, LPCAT1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66044-1-PBS. Detection antibody: 66044-2-PBS. Standard:Ag9060. Range: 0.391-100 ng/mL.

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  Cytometric bead array standard curve of MP50776-2

  Cytometric bead array standard curve of MP50776-2, LPCAT1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66044-1-PBS. Detection antibody: 66044-3-PBS. Standard:Ag9060. Range: 0.391-25 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66044-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA LPCAT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LPCAT1 fusion protein Ag9060
• Vollständiger Name: lysophosphatidylcholine acyltransferase 1
• Berechnetes Molekulargewicht: 534 aa, 59 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC020166
• Gene symbol: LPCAT1
• Gene ID (NCBI): 79888
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LPCAT1, also named as AYTL2, PFAAP3 and LysoPAFAT, belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family. It is a key enzyme for remodeling phospholipids, including phosphatidylcholine. The expression level of LPCAT1 is able to differentiate prostate cancer from noncancerous prostatic changes, and correlates to the tumor grade of prostate cancer. LPCAT1 possesses both acyltransferase and acetyltransferase activities. It mediates the conversion of 1-acyl-sn-glycero-3-phosphocholine (LPC) into phosphatidylcholine (PC).