LPCAT4 Polyklonaler Antikörper

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• KD/KO Validated

LPCAT4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 17905-1-AP

Synonyme

1-AGP acyltransferase 7, 1-acylglycerophosphoserine O-acyltransferase, 1-acylglycerophosphocholine O-acyltransferase, 1-acylglycerol-3-phosphate O-acyltransferase 7, 1 AGPAT 7

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  WB analysis using 17905-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17905-1-AP (LPCAT4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17905-1-AP

  WB result of LPCAT4 antibody (17905-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LPCAT4 transfected HeLa cells.

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  IHC staining of human intrahepatic cholangiocarcinoma using 17905-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 17905-1-AP (LPCAT4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 17905-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 17905-1-AP (LPCAT4 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 17905-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17905-1-AP (LPCAT4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 17905-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17905-1-AP (LPCAT4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-29-Zellen, HeLa-Zellen, HepG2-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

17905-1-AP bindet in WB, IHC, ELISA LPCAT4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LPCAT4 fusion protein Ag12281
• Vollständiger Name: lysophosphatidylcholine acyltransferase 4
• Berechnetes Molekulargewicht: 524 aa, 57 kDa
• Beobachtetes Molekulargewicht: 50-57 kDa
• GenBank-Zugangsnummer: BC092463
• Gene symbol: LPCAT4
• Gene ID (NCBI): 254531
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LPCAT4, also known as lysophosphatidylcholine acyltransferase 4, is an enzyme that plays a crucial role in lipid metabolism and membrane remodeling It is a member of the 1-acylglycerol-3-phosphate O-acyltransferase (EC 2.3.1.51) family and is involved in the conversion of lysophosphatidic acid (LPA) to phosphatidic acid (PA), a precursor in the biosynthesis of all glycerolipids. Both LPA and PA are involved in signal transduction. The protein is predicted to be located in the membrane and is classified as a metabolic protein and enzyme. It is involved in biological processes such as lipid biosynthesis, lipid metabolism, phospholipid biosynthesis, and phospholipid metabolism. .

Publikationen

Species	Application	Title
human	WB	J Hepatol; Lysophosphatidylcholine acyltransferase 1 altered phospholipid composition and regulated hepatoma progression.; Authors - Morita Yoshifumi Y
mouse	WB	Free Radic Biol Med; Role of ferroptosis mediated by abnormal membrane structure in DEHP-induced reproductive injury; Authors - Wei Deng
mouse	WB	Neurochem Res; iPLA2β Knockout Mouse, a Genetic Model for Progressive Human Motor Disorders, Develops Age-Related Neuropathology.; Authors - Helene Blanchard
human	WB	FASEB J; Deep multilayer brain omics identifies the potential involvement of menopause molecular networks in Gliomas' disease progression; Authors - Chunhua Liu
human	WB	Front Biosci (Landmark Ed); Upregulation of LPGAT1 Enhances Lung Adenocarcinoma Proliferation; Authors - Huiyuan Gong
mouse	WB	FASEB J; Overexpression of lysophospholipid acyltransferase, LPLAT10/LPCAT4/LPEAT2, in the mouse liver increases glucose-stimulated insulin secretion; Authors - Kahori Shimizu