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LPP Rekombinanter Antikörper

LPP Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250773C9

Kat-Nr. : 86117-3-PBS

Synonyme

LIM domain-containing preferred translocation partner in lipoma, Lipoma preferred partner, Lipoma-preferred partner

Filter:
  • Western Blot (WB) analysis of various lysates using LPP Recombinant antibody (86117-3-RR)

    WB analysis using 86117-3-RR (same clone as 86117-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86117-3-RR (LPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86117-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using LPP Recombinant antibody (86117-3-RR)

    WB analysis using 86117-3-RR (same clone as 86117-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86117-3-RR (LPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86117-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using LPP Recombinant antibody (86117-3-RR)

    IF Staining of HeLa using 86117-3-RR (same clone as 86117-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LPP antibody (86117-3-RR, Clone: 250773C9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86117-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86117-3-RR

    Affinity and Kinetic Characterization of 86117-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86117-3-RR against Human LPP were performed. The affinity constant is 1.18 nM.

Geprüfte Anwendungen

Produktinformation

86117-3-PBS bindet in WB, IF/ICC, Indirect ELISA LPP und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen LPP fusion protein Ag19205
Vollständiger Name LIM domain containing preferred translocation partner in lipoma
Berechnetes Molekulargewicht 612 aa, 66 kDa
Beobachtetes Molekulargewicht 70-75 kDa
GenBank-ZugangsnummerBC130584
Gene symbol LPP
Gene ID (NCBI) 4026
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LPP is a member of a subfamily of LIM domain proteins. Its characteristic is that the N-terminal region is rich in proline, and it has three C-terminal LIM domains. LPP localizes to the cell periphery in focal adhesions and may be involved in cell-cell adhesion and cell motility(PMID: 10637295). LPP is located at the junction of certain disease-related chromosomal translocations, which result in the expression of chimeric proteins that may promote tumor growth(PMID: 12581855).