LPP Rekombinanter Antikörper

LPP Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250773C9

Kat-Nr. : 86117-3-RR

Synonyme

LIM domain-containing preferred translocation partner in lipoma, Lipoma preferred partner, Lipoma-preferred partner

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  WB analysis using 86117-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86117-3-RR (LPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86117-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86117-3-RR (LPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86117-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LPP antibody (86117-3-RR, Clone: 250773C9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86117-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86117-3-RR against Human LPP were performed. The affinity constant is 1.18 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, MCF-7-Zellen, Maus-Kolongewebe, Ratten-Kolongewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86117-3-RR bindet in WB, IF/ICC, ELISA LPP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: LPP fusion protein Ag19205
• Vollständiger Name: LIM domain containing preferred translocation partner in lipoma
• Berechnetes Molekulargewicht: 612 aa, 66 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC130584
• Gene symbol: LPP
• Gene ID (NCBI): 4026
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LPP is a member of a subfamily of LIM domain proteins. Its characteristic is that the N-terminal region is rich in proline, and it has three C-terminal LIM domains. LPP localizes to the cell periphery in focal adhesions and may be involved in cell-cell adhesion and cell motility(PMID: 10637295). LPP is located at the junction of certain disease-related chromosomal translocations, which result in the expression of chimeric proteins that may promote tumor growth(PMID: 12581855).