LPXN Polyklonaler Antikörper

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• KD/KO Validated

LPXN Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 11307-1-AP

Synonyme

LDPL, LDLP

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  WB analysis using 11307-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11307-1-AP (LPXN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 11307-1-AP

  WB result of LPXN antibody (11307-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LPXN transfected PC-3 cells.

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  IP experiment of PC-3 using 11307-1-AP

  IP result of anti-LPXN (IP:11307-1-AP, 4ug; Detection:11307-1-AP 1:5000) with PC-3 cells lysate 1575 ug.

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  IF Staining of PC-3 using 11307-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using LPXN antibody (11307-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, PC-3-Zellen
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11307-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA LPXN und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LPXN fusion protein Ag1845
• Vollständiger Name: leupaxin
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC019035
• Gene symbol: LPXN
• Gene ID (NCBI): 9404
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LPXN, also known as Leupaxin, LDPL, belongs to the paxillin family. LPXN Contributes to the regulation of cell adhesion, spreading, and cell migration and acts as a negative regulator in integrin-mediated cell adhesion events (PMID: 20543562). LPXN has four leucine-rich LD-motifs in the N-terminus and four LIM domains in the C-terminus. It may function in cell type-specific signaling by associating with PYK2, a member of the focal adhesion kinase family (PMID: 9565592). Studies in breast cancer implicate LPXN is involved in the regulation of ER action as a co-factor (PMID: 25955236). LPXN is expressed in prostate cancer cells and its expression intensity is directly linked to PCa progression (PMID: 18451096).

Publikationen

Species	Application	Title
mouse	IF	J Mol Cell Cardiol; Exogenous extracellular matrix proteins decrease cardiac fibroblast activation in stiffening microenvironment through CAPG.; Authors - Xinming Wang
mouse	IHC	Mol Cell Biochem; STAT3 mediates ECM stiffness-dependent progression in ovarian cancer; Authors - Sun Chenchen