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LPXN Rekombinanter Antikörper

LPXN Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240483G10

Kat-Nr. : 83657-5-PBS

Synonyme

240483G10, LDLP, LDPL

Filter:
  • Western Blot (WB) analysis of various lysates using LPXN Recombinant antibody (83657-5-RR)

    WB analysis using 83657-5-RR (same clone as 83657-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83657-5-RR (LPXN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83657-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using LPXN Recombinant antibody (83657-5-RR)

    WB analysis of PC-3 using 83657-5-RR (same clone as 83657-5-PBS)

    WB result of LPXN antibody (83657-5-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-LPXN transfected PC-3 cells. This data was developed using the same antibody clone with 83657-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using LPXN Recombinant antibody (83657-5-RR)

    IF Staining of PC-3 using 83657-5-RR (same clone as 83657-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using LPXN antibody (83657-5-RR, Clone: 240483G10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83657-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83657-5-RR

    Affinity and Kinetic Characterization of 83657-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83657-5-RR against Human LPXN were performed. The affinity constant is 0.187 nM.

Geprüfte Anwendungen

Produktinformation

83657-5-PBS bindet in WB, IF/ICC, ELISA LPXN und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen LPXN fusion protein Ag34956
Vollständiger Name leupaxin
Berechnetes Molekulargewicht 43 kDa
Beobachtetes Molekulargewicht 45 kDa
GenBank-ZugangsnummerBC019035
Gene symbol LPXN
Gene ID (NCBI) 9404
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LPXN, also known as Leupaxin, LDPL, belongs to the paxillin family. LPXN Contributes to the regulation of cell adhesion, spreading, and cell migration and acts as a negative regulator in integrin-mediated cell adhesion events (PMID: 20543562). LPXN has four leucine-rich LD-motifs in the N-terminus and four LIM domains in the C-terminus. It may function in cell type-specific signaling by associating with PYK2, a member of the focal adhesion kinase family (PMID: 9565592). Studies in breast cancer implicate LPXN is involved in the regulation of ER action as a co-factor (PMID: 25955236). LPXN is expressed in prostate cancer cells and its expression intensity is directly linked to PCa progression (PMID: 18451096).