LPXN Rekombinanter Antikörper

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• KD/KO Validated

LPXN Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240483G10

Kat-Nr. : 83657-5-RR

Synonyme

240483G10, LDLP, LDPL

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  WB analysis using 83657-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83657-5-RR (LPXN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 83657-5-RR

  WB result of LPXN antibody (83657-5-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-LPXN transfected PC-3 cells.

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  IF Staining of PC-3 using 83657-5-RR

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using LPXN antibody (83657-5-RR, Clone: 240483G10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83657-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83657-5-RR against Human LPXN were performed. The affinity constant is 0.187 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Ramos-Zellen, Jurkat-Zellen, MDA-MB-231-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IF/ICC: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83657-5-RR bindet in WB, IF/ICC, ELISA LPXN und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: LPXN fusion protein Ag34956
• Vollständiger Name: leupaxin
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC019035
• Gene symbol: LPXN
• Gene ID (NCBI): 9404
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LPXN, also known as Leupaxin, LDPL, belongs to the paxillin family. LPXN Contributes to the regulation of cell adhesion, spreading, and cell migration and acts as a negative regulator in integrin-mediated cell adhesion events (PMID: 20543562). LPXN has four leucine-rich LD-motifs in the N-terminus and four LIM domains in the C-terminus. It may function in cell type-specific signaling by associating with PYK2, a member of the focal adhesion kinase family (PMID: 9565592). Studies in breast cancer implicate LPXN is involved in the regulation of ER action as a co-factor (PMID: 25955236). LPXN is expressed in prostate cancer cells and its expression intensity is directly linked to PCa progression (PMID: 18451096).