LRAT Polyklonaler Antikörper

LRAT Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 12815-1-AP

Synonyme

LRAT

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Galerie der Validierungsdaten

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  WB analysis of mouse testis using 12815-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12815-1-AP (LRAT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12815-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12815-1-AP (LRAT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Y79-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

12815-1-AP bindet in WB, ELISA LRAT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LRAT fusion protein Ag3556
• Vollständiger Name: lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase)
• Berechnetes Molekulargewicht: 230 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa, 50-54 kDa
• GenBank-Zugangsnummer: BC031053
• Gene symbol: LRAT
• Gene ID (NCBI): 9227
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LRAT belongs to the H-rev107 family. LRAT transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, LRAT monomers (26kDa) interact in membranes and form functional homodimers (50-54 kDa) through protein-protein interactions and disulfide bond formation.Defects in LRAT are a cause of severe early-onset retinal dystrophy (RD). This antibody was generated against the internal residues of human LRAT. It is predicted to detect endogenous LRAT.

Publikationen

Species	Application	Title
rat	WB	iScience; Control by the brain of vitamin A homeostasis; Authors - Peter I Imoesi
rat	WB	Am J Pathol; Impaired Rhodopsin Generation in the Rat Model of Diabetic Retinopathy.; Authors - Volha V Malechka
mouse	WB	J Food Biochem; Inhibitory effect of chitooligosaccharides on retinol metabolism and bioavailability in mice.; Authors - Wei Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Peter (Verified Customer) (12-03-2018)

The Proteintech LRAT primary antibody works so well and the antibody is prepared using BSA it can be use repeatedly when stored at -20. The strength of the antibody remains the same over a period of 6 months or more.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Rat Liver Tissue

LRAT Antibody Western Blot, validation (1:500 dilution) in Rat Liver Tissue (Cat no:12815-1-AP)