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LRAT Polyklonaler Antikörper

LRAT Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12815-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of mouse testis tissue using LRAT Polyclonal antibody (12815-1-AP)

    WB analysis of mouse testis using 12815-1-AP (same clone as 12815-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12815-1-AP (LRAT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12815-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using LRAT Polyclonal antibody (12815-1-AP)

    WB analysis of mouse testis using 12815-1-AP (same clone as 12815-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12815-1-AP (LRAT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12815-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Y79 cells using LRAT Polyclonal antibody (12815-1-AP)

    WB analysis of Y79 using 12815-1-AP (same clone as 12815-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 12815-1-AP (LRAT Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12815-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

12815-1-PBS bindet in WB, Indirect ELISA LRAT und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen LRAT fusion protein Ag3556
Vollständiger Name lecithin retinol acyltransferase (phosphatidylcholine--retinol O-acyltransferase)
Berechnetes Molekulargewicht 230 aa, 26 kDa
Beobachtetes Molekulargewicht 26 kDa, 50-54 kDa
GenBank-ZugangsnummerBC031053
Gene symbol LRAT
Gene ID (NCBI) 9227
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LRAT belongs to the H-rev107 family. LRAT transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters. LRAT plays a critical role in vision. It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, LRAT monomers (26kDa) interact in membranes and form functional homodimers (50-54 kDa) through protein-protein interactions and disulfide bond formation.Defects in LRAT are a cause of severe early-onset retinal dystrophy (RD). This antibody was generated against the internal residues of human LRAT. It is predicted to detect endogenous LRAT.