LRFN5 Rekombinanter Antikörper

LRFN5 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241060F5

Kat-Nr. : 83880-5-RR

Synonyme

241060F5, C14orf146, FIGLER8, Leucine-rich repeat and fibronectin type-III domain-containing protein 5, SALM5

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  WB analysis using 83880-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83880-5-RR (LRFN5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 83880-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83880-5-RR (LRFN5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 83880-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83880-5-RR (LRFN5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83880-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83880-5-RR against Human LRFN5 were performed. The affinity constant is 2.95 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83880-5-RR bindet in WB, IHC, ELISA LRFN5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: LRFN5 fusion protein Ag22152
• Vollständiger Name: leucine rich repeat and fibronectin type III domain containing 5
• Berechnetes Molekulargewicht: 719 aa, 79 kDa
• Beobachtetes Molekulargewicht: 79 kDa
• GenBank-Zugangsnummer: BC043165
• Gene symbol: LRFN5
• Gene ID (NCBI): 145581
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Leucine-rich repeat and fibronectin type-III domain-containing protein 5(LRFN5), belongs to a family of five small transmembrane protein genes involved in the development, organization, and plasticity of synapses. LRFN5 has another synonym which stands for synaptic adhesion-like molecule 5 (SALM5). SALMs are newly characterized adhesion molecules predominantly expressed in the brain contributing to neurite outgrowth and synapse formation. (PMID: 37280213)