LRP5 Polyklonaler Antikörper

LRP5 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24899-1-PBS

Synonyme

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  WB analysis of HeLa using 24899-1-AP (same clone as 24899-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 24899-1-AP (LRP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24899-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 24899-1-AP (same clone as 24899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 24899-1-AP (LRP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24899-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 24899-1-AP (same clone as 24899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 24899-1-AP (LRP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24899-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 24899-1-AP (same clone as 24899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24899-1-AP (LRP5 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24899-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 24899-1-AP (same clone as 24899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24899-1-AP (LRP5 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24899-1-PBS in a different storage buffer formulation.

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  IHC staining of human hepatocirrhosis using 24899-1-AP (same clone as 24899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24899-1-AP (LRP5 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24899-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human hepatocirrhosis using 24899-1-AP (same clone as 24899-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24899-1-AP (LRP5 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24899-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 24899-1-AP (same clone as 24899-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 24899-1-AP (LRP5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24899-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24899-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA LRP5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LRP5 fusion protein Ag19034
• Vollständiger Name: low density lipoprotein receptor-related protein 5
• Berechnetes Molekulargewicht: 1615 aa, 179 kDa
• Beobachtetes Molekulargewicht: 180-200 kDa
• GenBank-Zugangsnummer: BC150595
• Gene symbol: LRP5
• Gene ID (NCBI): 4041
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Low-density lipoprotein receptor-related protein 5 (LRP5) is a single-pass type I membrane protein with EGF-like and LDLR domains in the extracellular domain. LRP5 and LRP6 act as coreceptors with Frizzled for Wnt. The complex of Wnt-Fzd-LRP5-LRP6 triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. LRP5 plays a key role in skeletal homeostasis and many bone density related diseases are caused by mutations in LRP5 gene.