LRPPRC Monoklonaler Antikörper

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LRPPRC Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3H7E1

Kat-Nr. : 67679-1-PBS

Synonyme

130 kDa leucine-rich protein, 3H7E1, GP130, Leucine-rich PPR motif-containing protein, mitochondrial, LRP 130

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  WB analysis using 67679-1-Ig (same clone as 67679-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67679-1-Ig (LRPPRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67679-1-Ig (same clone as 67679-1-PBS)

  WB result of LRPPRC antibody (67679-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LRPPRC transfected HeLa cells. This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

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  WB analysis of U-251 using 67679-1-Ig (same clone as 67679-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 67679-1-Ig (LRPPRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67679-1-Ig (same clone as 67679-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67679-1-Ig (same clone as 67679-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 67679-1-Ig (same clone as 67679-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

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  IHC staining of human endometrial cancer using 67679-1-Ig (same clone as 67679-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67679-1-Ig (same clone as 67679-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67679-1-Ig (same clone as 67679-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67679-1-Ig (LRPPRC antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67679-1-Ig (same clone as 67679-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67679-1-Ig (same clone as 67679-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 67679-1-Ig (same clone as 67679-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). F-actin is stained using CoraLite®555-phalloidin (red) and DNA is stained by DAPI (blue). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67679-1-Ig (same clone as 67679-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67679-1-Ig (same clone as 67679-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using LRPPRC antibody (67679-1-Ig, Clone: 3H7E1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67679-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67679-1-PBS bindet in WB, IHC, IF/ICC, ELISA LRPPRC und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LRPPRC fusion protein Ag30261
• Vollständiger Name: leucine-rich PPR-motif containing
• Berechnetes Molekulargewicht: 1394 aa, 158 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC050311
• Gene symbol: LRPPRC
• Gene ID (NCBI): 10128
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.