LRPPRC Rekombinanter Antikörper

LRPPRC Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251577F1

Kat-Nr. : 86616-2-PBS

Synonyme

130 kDa leucine-rich protein, GP130, Leucine-rich PPR motif-containing protein, mitochondrial, LRP 130, LRP130

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  WB analysis using 86616-2-RR (same clone as 86616-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86616-2-RR (LRPPRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86616-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02559-1

  The mean LRPPRC concentration was determined to be 181.06 ng/mL in HCT116 cell extract based on a 1.0 mg/mL extract load, 438.54 ng/mL in HepG2 cell extract based on a 1.0 mg/mL extract load and 207.78 ng/mL in SH-SY5Y cell extract based on a 1.0 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02559-1

  Sandwich ELISA standard curve of MP02559-1, Human LRPPRC Recombinant Matched Antibody Pair - PBS only. 86616-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag15452. 86616-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL

• 

  Cytometric bead array sample test of MP02559-1

  The mean LRPPRC concentration was determined to be 67.2 ng/mL in HeLa cell extract based on a 1.1 mg/mL extract load, 168.4 ng/mL in HCT 116 cell extract based on a 1.3 mg/mL extract load.

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  Cytometric bead array standard curve of MP02559-1

  Cytometric bead array standard curve of MP02559-1, LRPPRC Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86616-1-PBS. Detection antibody: 86616-2-PBS. Standard: Ag15452. Range: 1.562-100 ng/mL.

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  Affinity and Kinetic Characterization of 86616-2-RR

  Biolayer interferometry (BLl) kinetic assays of 86616-2-RR against Human LRPPRC were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

86616-2-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA LRPPRC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: LRPPRC fusion protein Ag15452
• Vollständiger Name: leucine-rich PPR-motif containing
• Berechnetes Molekulargewicht: 1394 aa, 158 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC050311
• Gene symbol: LRPPRC
• Gene ID (NCBI): 10128
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria, where it binds to poly(A) mRNA. It plays a role in the translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in the LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.