LRRC2 Rekombinanter Antikörper

LRRC2 Rekombinant Antikörper für IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241132G1

Kat-Nr. : 84404-1-PBS

Synonyme

Leucine-rich repeat-containing protein 2, 241132G1

---

• 

  IHC staining of mouse skeletal muscle using 84404-1-RR (same clone as 84404-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84404-1-RR (LRRC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84404-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 84404-1-RR (same clone as 84404-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84404-1-RR (LRRC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84404-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U-251 using 84404-1-RR (same clone as 84404-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using LRRC2 antibody (84404-1-RR, Clone: 241132G1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84404-1-PBS in a different storage buffer formulation.

• 

  FC experiment of U-251 using 84404-1-RR (same clone as 84404-1-PBS)

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug LRRC2 Recombinant antibody (84404-1-RR, Clone:241132G1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84404-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84404-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84404-1-RR against Human LRRC2 were performed. The affinity constant is 26.2 nM.

---

Geprüfte Anwendungen

Produktinformation

84404-1-PBS bindet in IHC, IF/ICC, FC (Intra), Indirect ELISA LRRC2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: LRRC2 fusion protein Ag27830
• Vollständiger Name: leucine rich repeat containing 2
• Berechnetes Molekulargewicht: 371 aa, 43 kDa
• GenBank-Zugangsnummer: BC029118
• Gene symbol: LRRC2
• Gene ID (NCBI): 79442
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Leucine Rich Repeat Containing 2 (LRRC2) was found to be localized to the mitochondria in human cells and transcriptionally regulated by the mitochondrial master regulator Pgc-1α. It has been reported that Lrrc2 transcript abundance correlates with that of β-MHC, a canonical marker of cardiac hypertrophy in humans, and experimentally demonstrated an elevation in Lrrc2 transcript in vitro and in vivo rodent models of cardiac hypertrophy as well as in patients with dilated cardiomyopathy. (PMID: 28158196)