LRRC32 Polyklonaler Antikörper

LRRC32 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (1)

Kat-Nr. : 26021-1-AP

Synonyme

D11S833E, GARP, Garpin, LRRC32

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  WB analysis of HUVEC using 26021-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 26021-1-AP (LRRC32 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 26021-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26021-1-AP (LRRC32 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 26021-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26021-1-AP (LRRC32 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 26021-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26021-1-AP (LRRC32 Antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 26021-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26021-1-AP (LRRC32 Antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

26021-1-AP bindet in WB, IHC, ELISA LRRC32 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LRRC32 fusion protein Ag23423
• Vollständiger Name: leucine rich repeat containing 32
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC070079
• Gene symbol: LRRC32
• Gene ID (NCBI): 2615
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LRRC32, also known as GARP, is a transmembrane protein of 662 amino acids, the extracellular portion of which contains 20 leucine-rich repeats (PMID: 8180135). LRRC32 is a cell surface receptor on regulatory T-lymphocytes (Treg cells), platelets, hepatic stellate cells and certain cancer cells (PMID: 27054568). It has been demonstrated as a Treg-specific activation marker (PMID: 20137067). LRRC32 is critical for the surface expression of latent TGF-β by binding to the complex and functioning as its cell surface receptor (PMID: 19651619).

Publikationen

Species	Application	Title
mouse	WB	iScience; GRP94 promotes anoikis resistance and peritoneal metastasis through YAP/TEAD1 pathway in gastric cancer; Authors - Qimeng Shi

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Martina (Verified Customer) (07-09-2021)

Antibody worked well for WB and IHC, specificity confirmed by knockdown and over-extpression

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:500 WB 1:5000 IHC
• Cell Tissue Type: Human Skin