LRRC45 Polyklonaler Antikörper

LRRC45 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32111-1-AP

Synonyme

LRC45, Leucine-rich repeat-containing protein 45

---

• 

  WB analysis using 32111-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32111-1-AP (LRRC45 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A431 using 32111-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using LRRC45 antibody (32111-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of U-251 using 32111-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using LRRC45 antibody (32111-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, HeLa-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IF/ICC: A431-Zellen, U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

32111-1-AP bindet in WB, IF/ICC, ELISA LRRC45 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LRRC45 fusion protein Ag36776
• Vollständiger Name: leucine rich repeat containing 45
• Berechnetes Molekulargewicht: 76 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC014109
• Gene symbol: LRRC45
• Gene ID (NCBI): 201255
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Leucine-Rich Repeat-Containing 45 (LRRC45) gene, a relatively unexplored entity in the realm of genomic studies, has recently garnered attention for its potential role in cellular processes and disease pathogenesis. Characterized by its leucine-rich repeats, a motif known for facilitating protein-protein interactions, LRRC45 is implicated in various cellular functions, including signal transduction and intracellular trafficking. LRRC45 promotes lung cancer proliferation and progression by enhancing c-MYC, Slug, MMP2, and MMP9 expression (PMID: 39326735).