LRRC47 Polyklonaler Antikörper

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• KD/KO Validated

LRRC47 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 23217-1-AP

Synonyme

Leucine-rich repeat-containing protein 47, KIAA1185

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  WB analysis of mouse liver using 23217-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 23217-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 23217-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 23217-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 23217-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 23217-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 23217-1-AP (LRRC47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas using 23217-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23217-1-AP (LRRC47 Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas using 23217-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 23217-1-AP (LRRC47 Antibody) at dilution of 1:50.

• 

  IF Staining of L02 using 23217-1-AP

  Immunofluorescent analysis of L02 cells using 23217-1-AP (LRRC47 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, L02-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: L02-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

23217-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA LRRC47 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LRRC47 fusion protein Ag19736
• Vollständiger Name: leucine rich repeat containing 47
• Berechnetes Molekulargewicht: 583 aa, 63 kDa
• Beobachtetes Molekulargewicht: 63 kDa
• GenBank-Zugangsnummer: BC031301
• Gene symbol: LRRC47
• Gene ID (NCBI): 57470
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LRRC47 enables RNA binding activity. It is predicted to be involved in phenylalanyl-tRNA aminoacylation.

Publikationen

Species	Application	Title
human	WB	Nature; Structural basis for the final steps of human 40S ribosome maturation.; Authors - Michael Ameismeier ; KD Validated
human	WB	bioRxiv; LARP1 senses free ribosomes to coordinate supply and demand of ribosomal proteins; Authors - James A Saba ; KD Validated
human	WB,CoIP,IHC	Cell Rep; The C9ORF72 repeat expansion alters neurodevelopment; Authors - Eric Hendricks