LRRC47 Rekombinanter Antikörper

LRRC47 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240741A6

Kat-Nr. : 83693-3-RR

Synonyme

Leucine-rich repeat-containing protein 47, KIAA1185, 240741A6

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  WB analysis using 83693-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83693-3-RR (LRRC47 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of L02 using 83693-3-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using LRRC47 antibody (83693-3-RR, Clone: 240741A6 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of U2OS using 83693-3-RR

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug LRRC47 Recombinant antibody (83693-3-RR, Clone:240741A6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83693-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83693-3-RR against Human LRRC47 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Daudi-Zellen, L02-Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IF/ICC: L02-Zellen
• Erfolgreiche Detektion in FC (Intra): U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83693-3-RR bindet in WB, IF/ICC, FC (Intra), ELISA LRRC47 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: LRRC47 fusion protein Ag19736
• Vollständiger Name: leucine rich repeat containing 47
• Berechnetes Molekulargewicht: 583 aa, 63 kDa
• Beobachtetes Molekulargewicht: 63 kDa
• GenBank-Zugangsnummer: BC031301
• Gene symbol: LRRC47
• Gene ID (NCBI): 57470
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LRRC47 enables RNA binding activity. It is predicted to be involved in phenylalanyl-tRNA aminoacylation.