LRRC8A/SWELL1 Polyklonaler Antikörper

LRRC8A/SWELL1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17155-1-PBS

Synonyme

LRRC8A, SWELL1, LRRC8, Leucine-rich repeat-containing protein 8A, KIAA1437

---

• 

  WB analysis using 17155-1-AP (same clone as 17155-1-PBS)

  rat lung tissue were subjected to SDS PAGE followed by western blot with 17155-1-AP (LRRC8A/SWELL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17155-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse brain using 17155-1-AP (same clone as 17155-1-PBS)

  IP result of anti-LRRC8A/SWELL1 (IP:17155-1-AP, 4ug; Detection:17155-1-AP 1:800) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 17155-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 17155-1-AP (same clone as 17155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17155-1-AP (LRRC8A/SWELL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17155-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 17155-1-AP (same clone as 17155-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17155-1-AP (LRRC8A/SWELL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17155-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

17155-1-PBS bindet in WB, IHC, IP, Indirect ELISA LRRC8A/SWELL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LRRC8A/SWELL1 fusion protein Ag10062
• Vollständiger Name: leucine rich repeat containing 8 family, member A
• Berechnetes Molekulargewicht: 810 aa, 94 kDa
• Beobachtetes Molekulargewicht: 94 kDa
• GenBank-Zugangsnummer: BC051322
• Gene symbol: LRRC8A
• Gene ID (NCBI): 56262
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LRRC8A, also named as SWELL1, KIAA1437 and LRRC8, belongs to the LRRC8 family. LRRC8A is a ubiquitous and integral component of the VRAC and has been reported to be required for regulation of cell volume. It indicates that LRRC8A plays a role in regulating the flow of ions, especially anions, into and out of cells (PMID: 32079488). Additionally, there have been several reports that LRRC8A is critical for differentiation of adipocytes and lymphocytes (PMID: 31323188). The molecular weight of LRRC8A is 94 kDa.