LRRC8C Polyklonaler Antikörper

LRRC8C Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 21601-1-AP

Synonyme

Volume-regulated anion channel subunit LRRC8C, Leucine-rich repeat-containing protein 8C, FAD158, AD158



Geprüfte Anwendungen

Erfolgreiche Detektion in WBTHP-1-Zellen
Erfolgreiche Detektion in IHCMausherzgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

WBSee 6 publications below
IFSee 2 publications below

Produktinformation

21601-1-AP bindet in WB, IHC, IF/ICC, ELISA LRRC8C und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen LRRC8C fusion protein Ag16213
Vollständiger Name leucine rich repeat containing 8 family, member C
Berechnetes Molekulargewicht 803 aa, 92 kDa
Beobachtetes Molekulargewicht92 kDa
GenBank-ZugangsnummerBC113973
Gene symbol LRRC8C
Gene ID (NCBI) 84230
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LRRC8C (Leucine-rich repeat-containing protein 8C), also known as AD158, is a non-essential component of the volume-regulated anion channel (VRAC, also known as VSOAC channel), which is required to maintain a constant cell volume in response to extracellular or intracellular permeability changes. The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine. The VRAC channel also mediates transport of immunoreactive cyclic dinucleotide GMP-AMP (2'-3'-cGAMP), an immune messenger produced in response to DNA virus in the cytosol (PMID:24790029; 26824658; 28193731).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for LRRC8C antibody 21601-1-APProtokoll herunterladen
IHC protocol for LRRC8C antibody 21601-1-APProtokoll herunterladenl
IF protocol for LRRC8C antibody 21601-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Nat Commun

TET2-mediated tumor cGAS triggers endothelial STING activation to regulate vasculature remodeling and anti-tumor immunity in liver cancer

Authors - Hongwei Lv
ratIF

Diabetes Metab J

Single-Cell Landscape and a Macrophage Subset Enhancing Brown Adipocyte Function in Diabetes

Authors - Junfei Gu
humanWB

bioRxiv

LRRC8 complexes are adenosine nucleotide release channels regulating platelet activation and arterial thrombosis

Authors - John D Tranter
mouseWB

bioRxiv

Lysosomal LRRC8 complex regulates lysosomal pH, morphology and systemic glucose metabolism

Authors - Ashutosh Kumar
humanWB

Sci Adv

2'3'-cGAMP interactome identifies 2'3'-cGAMP/Rab18/FosB signaling in cell migration control independent of innate immunity

Authors - Yu Deng
humanWB

Sci Adv

Regulation of volume-regulated anion channels alters sensitivity to platinum chemotherapy

Authors - Lily Elizabeth R Feldman

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Joshua (Verified Customer) (08-16-2021)

This antibody was incubated at 4 degrees C O/N, in 5% Milk, and at a dilution ratio of 1:1,000. A secondary antibody at a dilution ratio of 1:5,000 was used. The acquired western blot image was achieved at an exposure time of 60 seconds. This product was vital in visualizing LRRC8c in both HUVECs and mouse lung tissue.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: HUVECs and Mouse Lung Tissue