LRRC8C Monoklonaler Antikörper

LRRC8C Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4A6A4

Kat-Nr. : 68323-1-PBS

Synonyme

AD158, DKFZp586J1119, FAD158, LRRC8C

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  WB analysis of U2OS using 68323-1-Ig (same clone as 68323-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 68323-1-Ig (LRRC8C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68323-1-PBS in a different storage buffer formulation.

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  WB analysis using 68323-1-Ig (same clone as 68323-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68323-1-Ig (LRRC8C antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68323-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68323-1-Ig (same clone as 68323-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using LRRC8C antibody (68323-1-Ig, Clone: 4A6A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68323-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68323-1-PBS bindet in WB, IF/ICC, Indirect ELISA LRRC8C und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LRRC8C fusion protein Ag17001
• Vollständiger Name: leucine rich repeat containing 8 family, member C
• Berechnetes Molekulargewicht: 803 aa, 92 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC113973
• Gene symbol: LRRC8C
• Gene ID (NCBI): 84230
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LRRC8C (Leucine-rich repeat-containing protein 8C), also known as AD158, is a non-essential component of the volume-regulated anion channel (VRAC, also known as VSOAC channel), which is required to maintain a constant cell volume in response to extracellular or intracellular permeability changes. The VRAC channel conducts iodide better than chloride and can also conduct organic osmolytes like taurine. The VRAC channel also mediates transport of immunoreactive cyclic dinucleotide GMP-AMP (2'-3'-cGAMP), an immune messenger produced in response to DNA virus in the cytosol (PMID:24790029; 26824658; 28193731).