LRRC8D Polyklonaler Antikörper

LRRC8D Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11537-1-PBS

Synonyme

Volume-regulated anion channel subunit LRRC8D, LRRC5, Leucine-rich repeat-containing protein 8D, Leucine-rich repeat-containing protein 5, HsLRRC8D

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  WB analysis of mouse brain using 11537-1-AP (same clone as 11537-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11537-1-AP (LRRC8D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11537-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 11537-1-AP (same clone as 11537-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11537-1-AP (LRRC8D antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11537-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 11537-1-AP (same clone as 11537-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11537-1-AP (LRRC8D antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11537-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 11537-1-AP (same clone as 11537-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 11537-1-AP (LRRC8D antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11537-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11537-1-AP (same clone as 11537-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LRRC8D antibody (11537-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11537-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11537-1-PBS bindet in WB, IHC, IF/ICC, ELISA LRRC8D und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LRRC8D fusion protein Ag2138
• Vollständiger Name: leucine rich repeat containing 8 family, member D
• Berechnetes Molekulargewicht: 858 aa, 98 kDa
• Beobachtetes Molekulargewicht: 98-120 kDa
• GenBank-Zugangsnummer: BC024159
• Gene symbol: LRRC8D
• Gene ID (NCBI): 55144
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LRRC8D (or LRRC5L) gene encodes a 98-kDa multi-pass membrane protein leucine-rich repeat-containing protein 8D, which contains 13 LRRs (leucine-rich repeats). The molecular function of this protein is so far not well understood. Recently, LRRC8D gene was found to be up-regulated after IL-2 deprivation in immortalised Interleukin-2(IL-2)-dependent human T cells, indicating a possible involvement of LRRC8D in cancer development. This LRRC8D antibody can't cross-recognize LRRC8A.