LRRC8D Rekombinanter Antikörper

LRRC8D Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251257E6

Kat-Nr. : 86510-1-PBS

Synonyme

HsLRRC8D, Leucine-rich repeat-containing protein 5, Leucine-rich repeat-containing protein 8D, LRRC5, Volume-regulated anion channel subunit LRRC8D

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  WB analysis of HaCaT using 86510-1-RR (same clone as 86510-1-PBS)

  HaCaT cells were subjected to SDS PAGE followed by western blot with 86510-1-RR (LRRC8D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86510-1-PBS in a different storage buffer formulation.

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  WB analysis using 86510-1-RR (same clone as 86510-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86510-1-RR (LRRC8D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86510-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 86510-1-RR (same clone as 86510-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using LRRC8D antibody (86510-1-RR, Clone: 251257E6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86510-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

86510-1-PBS bindet in WB, IF/ICC, Indirect ELISA LRRC8D und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: LRRC8D fusion protein Ag2138
• Vollständiger Name: leucine rich repeat containing 8 family, member D
• Berechnetes Molekulargewicht: 858 aa, 98 kDa
• Beobachtetes Molekulargewicht: 98-120 kDa
• GenBank-Zugangsnummer: BC024159
• Gene symbol: LRRC8D
• Gene ID (NCBI): 55144
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LRRC8D (or LRRC5L) gene encodes a 98-kDa multi-pass membrane protein leucine-rich repeat-containing protein 8D, which contains 13 LRRs (leucine-rich repeats). The molecular function of this protein is so far not well understood. Recently, LRRC8D gene was found to be up-regulated after IL-2 deprivation in immortalised Interleukin-2(IL-2)-dependent human T cells, indicating a possible involvement of LRRC8D in cancer development. This LRRC8D antibody can't cross-recognize LRRC8A.