LRRFIP1 Rekombinanter Antikörper

LRRFIP1 Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242161F10

Kat-Nr. : 84778-2-PBS

Synonyme

242161F10, FLAP 1, FLIIAP1, GC binding factor 2, GC-binding factor 2

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  WB analysis using 84778-2-RR (same clone as 84778-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84778-2-RR (LRRFIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84778-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01600-1

  Sandwich ELISA standard curve of MP01600-1, Human LRRFIP1 Recombinant Matched Antibody Pair - PBS only. 84778-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag34760. 84778-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-2500 pg/mL

• 

  Sample test of MP01600-1

  The mean LRRFIP1 concentration was determined to be 146.23 ng/mL in Raji cell extract based on a 1.4 mg/mL extract load, 70.95 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load and 131.01 ng/mL in A549 cell extract based on a 2.5 mg/mL extract load.

• 

  Cytometric bead array standard curve of MP01600-1

  Cytometric bead array standard curve of MP01600-1, LRRFIP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84778-1-PBS. Detection antibody: 84778-2-PBS. Standard: Ag34760. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84778-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84778-2-RR against Human LRRFIP1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84778-2-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test LRRFIP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: LRRFIP1 fusion protein Ag34760
• Vollständiger Name: leucine rich repeat (in FLII) interacting protein 1
• Beobachtetes Molekulargewicht: 150-170 kDa
• GenBank-Zugangsnummer: BC108914
• Gene symbol: LRRFIP1
• Gene ID (NCBI): 9208
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LRRFIP1 is a transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. The endogenous LRRFIP1/GCF2 proteins in several human cell lines, the one expressed from LRRFIP1/GCF2 cDNA in reticulolysates, and the recombinant LRRFIP1/GCF2 protein produced in bacteria have migrated as a band with a molecular weight (MW) of 160 kda in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).