LRRFIP1 Rekombinanter Antikörper

LRRFIP1 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242161C9

Kat-Nr. : 84778-4-PBS

Synonyme

242161C9, FLAP 1, FLIIAP1, GC binding factor 2, GC-binding factor 2

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  WB analysis using 84778-4-RR (same clone as 84778-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84778-4-RR (LRRFIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84778-4-PBS in a different storage buffer formulation.

• 

  WB analysis using 84778-4-RR (same clone as 84778-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84778-4-RR (LRRFIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84778-4-PBS in a different storage buffer formulation.

• 

  WB analysis using 84778-4-RR (same clone as 84778-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84778-4-RR (LRRFIP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84778-4-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 84778-4-RR (same clone as 84778-4-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using LRRFIP1 antibody (84778-4-RR, Clone: 242161C9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84778-4-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84778-4-PBS bindet in WB, IF/ICC, Indirect ELISA LRRFIP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: LRRFIP1 fusion protein Ag34760
• Vollständiger Name: leucine rich repeat (in FLII) interacting protein 1
• Beobachtetes Molekulargewicht: 170 kDa
• GenBank-Zugangsnummer: BC108914
• Gene symbol: LRRFIP1
• Gene ID (NCBI): 9208
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LRRFIP1 is a transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. The endogenous LRRFIP1/GCF2 proteins in several human cell lines, the one expressed from LRRFIP1/GCF2 cDNA in reticulolysates, and the recombinant LRRFIP1/GCF2 protein produced in bacteria have migrated as a band with a molecular weight (MW) of 160 kda in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).