LSM12 Monoklonaler Antikörper

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LSM12 Monoklonal Antikörper für WB, IHC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2E3G3

Kat-Nr. : 67029-1-PBS

Synonyme

Protein LSM12, 2E3G3

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  WB analysis using 67029-1-Ig (same clone as 67029-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67029-1-Ig (LSM12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67029-1-Ig (same clone as 67029-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67029-1-Ig (same clone as 67029-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67029-1-Ig (same clone as 67029-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67029-1-Ig (same clone as 67029-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67029-1-Ig (same clone as 67029-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67029-1-Ig (LSM12 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 67029-1-Ig (same clone as 67029-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug LSM12 Monoclonal antibody (67029-1-Ig, Clone:2E3G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67029-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67029-1-PBS bindet in WB, IHC, FC (Intra), Indirect ELISA LSM12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LSM12 fusion protein Ag28782
• Vollständiger Name: LSM12 homolog (S. cerevisiae)
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC044587
• Gene symbol: LSM12
• Gene ID (NCBI): 124801
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.