LSM3 Polyklonaler Antikörper

LSM3 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15879-1-PBS

Synonyme

YLR438C, USS2, U6 snRNA-associated Sm-like protein LSm3, SMX4, MDS017

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  WB analysis using 15879-1-AP (same clone as 15879-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15879-1-AP (LSM3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15879-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 15879-1-AP (same clone as 15879-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15879-1-AP (LSM3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15879-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 15879-1-AP (same clone as 15879-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15879-1-AP (LSM3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15879-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 15879-1-AP (same clone as 15879-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15879-1-AP (LSM3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15879-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 15879-1-AP (same clone as 15879-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15879-1-AP (LSM3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15879-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 15879-1-AP (same clone as 15879-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using LSM3 antibody (15879-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15879-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15879-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA LSM3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LSM3 fusion protein Ag8641
• Vollständiger Name: LSM3 homolog, U6 small nuclear RNA associated (S. cerevisiae)
• Berechnetes Molekulargewicht: 102 aa, 12 kDa
• Beobachtetes Molekulargewicht: 12 kDa
• GenBank-Zugangsnummer: BC007055
• Gene symbol: LSM3
• Gene ID (NCBI): 27258
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LSM3, as a U6 snRNA-associated Sm-like protein, plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex)