LSR Polyklonaler Antikörper

LSR Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18216-1-PBS

Synonyme

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  WB analysis of HT-29 using 18216-1-AP (same clone as 18216-1-PBS)

  HT-29 cells (LSR+) and HeLa cells (LSR-) were subjected to SDS PAGE followed by western blot with 18216-1-AP (LSR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18216-1-PBS in a different storage buffer formulation.

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  WB analysis using 18216-1-AP (same clone as 18216-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18216-1-AP (LSR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18216-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 18216-1-AP (same clone as 18216-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18216-1-AP (LSR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18216-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 18216-1-AP (same clone as 18216-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 18216-1-AP (LSR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18216-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 18216-1-AP (same clone as 18216-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 18216-1-AP (LSR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18216-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 18216-1-AP (same clone as 18216-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 18216-1-AP (LSR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18216-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18216-1-PBS bindet in WB, IHC, Indirect ELISA LSR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LSR fusion protein Ag12961
• Vollständiger Name: lipolysis stimulated lipoprotein receptor
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa, 56 kDa
• GenBank-Zugangsnummer: BC004381
• Gene symbol: LSR
• Gene ID (NCBI): 51599
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LSR (lipolysis stimulated lipoprotein receptor) is a lipoprotein receptor primarily expressed in the liver and activated by free fatty acids (PMID: 10224102). This remnant receptor binds both apoB- and apoE-containing lipoproteins and displays greatest affinity for TG-rich lipoproteins. Distribution of LSR causes embryonic lethality in mice, indicating that the expression of LSR is critical for liver and embryonic development (PMID: 15265030). Investigations show that LSR is a multimer consisting of α subunit (68 kDa) and β subunit (56 kDa) associated through disulfide bridges (PMID: 10224102; 19188430; 18644789).