LSS Polyklonaler Antikörper

LSS Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18693-1-PBS

Synonyme

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  WB analysis of HeLa using 18693-1-AP (same clone as 18693-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 18693-1-AP (LSS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18693-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 18693-1-AP (same clone as 18693-1-PBS)

  IP result of anti-LSS (IP:18693-1-AP, 4ug; Detection:18693-1-AP 1:500) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 18693-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 18693-1-AP (same clone as 18693-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18693-1-AP (LSS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18693-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 18693-1-AP (same clone as 18693-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18693-1-AP (LSS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18693-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 18693-1-AP (same clone as 18693-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 18693-1-AP (LSS antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18693-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18693-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA LSS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LSS fusion protein Ag6955
• Vollständiger Name: lanosterol synthase (2,3-oxidosqualene-lanosterol cyclase)
• Berechnetes Molekulargewicht: 732 aa, 83 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC035638
• Gene symbol: LSS
• Gene ID (NCBI): 4047
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LSS, also named as OSC, is lanosterol synthase which catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus. LSS gene has three isoforms with MW 74 kDa and 82-85 kDa. This antibody can recognize all these three isoforms.