LTA4H Polyklonaler Antikörper

LTA4H Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 13662-1-AP

Synonyme

EC:3.3.2.6, EC:3.4.11.4, Leukotriene A-4 hydrolase, LTA 4 hydrolase, LTA4

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  WB analysis using 13662-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13662-1-AP (LTA4H antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13662-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13662-1-AP (LTA4H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13662-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13662-1-AP (LTA4H antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13662-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13662-1-AP (LTA4H Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 13662-1-AP

  IP result of anti-LTA4H (IP:13662-1-AP, 4ug; Detection:13662-1-AP 1:500) with HEK-293 cells lysate 1120ug.

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  IHC staining of human skin using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 13662-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 13662-1-AP (LTA4H antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautgewebe, humanes Hirngewebe, humanes Herzgewebe, humanes Nierengewebe, humanes Lungengewebe, humanes Eierstockgewebe, humanes Plazenta-Gewebe, humanes Milzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

13662-1-AP bindet in WB, IHC, IF, IP, ELISA LTA4H und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LTA4H fusion protein Ag4568
• Vollständiger Name: leukotriene A4 hydrolase
• Berechnetes Molekulargewicht: 611 aa, 69 kDa
• Beobachtetes Molekulargewicht: 57-70 kDa
• GenBank-Zugangsnummer: BC032528
• Gene symbol: LTA4H
• Gene ID (NCBI): 4048
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LTA4H(Leukotriene A-4 hydrolase), also named as LTA4, is essential for the conversion of LTA4 to LTB4, an inflammatory lipid mediator(PMID:10601658). LTA4H also possesses aminopeptidase activity that it can degrade PGP, which is a biomarker for chronic obstructive pulmonary disease and is implicated in neutrophil persistence in the lung(PMID:20813919). This protein has 4 isoforms produced by alternative splicing with the MW of 69, 59, 57, 67 kDa.

Publikationen

Species	Application	Title
human	WB	Sci Signal; ALOX5 drives the pyroptosis of CD4+ T cells and tissue inflammation in rheumatoid arthritis; Authors - Hao Cai
human	WB	Proteomics; Mass Spectrometric Analysis Identifies AIMP1 and LTA4H as FSCN1-Binding Proteins in Laryngeal Squamous Cell Carcinoma.; Authors - Wei Gao
mouse	IF	JHEP Rep; Time-resolved single-cell transcriptomics reveals the landscape and dynamics of hepatic cells in sepsis-induced acute liver dysfunction; Authors - Gan Chen