LTBP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

LTBP1 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Rezensionen (1)

Kat-Nr. : 26855-1-AP

Synonyme

LTBP 1, LTBP1

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Galerie der Validierungsdaten

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  WB analysis of human plasma using 26855-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 26855-1-AP (LTBP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 26855-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26855-1-AP (LTBP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 26855-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26855-1-AP (LTBP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 26855-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 26855-1-AP (LTBP1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

26855-1-AP bindet in WB, IHC, IF, ELISA LTBP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LTBP1 fusion protein Ag25392
• Vollständiger Name: latent transforming growth factor beta binding protein 1
• Berechnetes Molekulargewicht: 1721 aa, 187 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC130289
• Gene symbol: LTBP1
• Gene ID (NCBI): 4052
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Latent TGFβ binding protein 1 (LTBP1) is a large extracellular protein which belongs to the family of latent TGF-beta binding proteins (LTBPs). LTBP1 is essential for TGF-beta folding, secretion, matrix localization and activation. LTBP1 has also been demonstrated to interact with a number of insoluble extracellular matrix components, such as fibrillin. We got 150-240 kDa in western blotting due to protein glycosylation of LTBP1 (PMID: 18086923, PMID: 9923648 ).

Publikationen

Species	Application	Title
human	IF	Cell Rep; Integrative single-cell meta-analysis reveals disease-relevant vascular cell states and markers in human atherosclerosis; Authors - Jose Verdezoto Mosquera
human	WB,IHC	J Transl Med; LTBP1 promotes esophageal squamous cell carcinoma progression through epithelial-mesenchymal transition and cancer-associated fibroblasts transformation.; Authors - Rui Cai ; KD Validated
human	WB	Cell Death Discov; ERRFI1 induces apoptosis of hepatocellular carcinoma cells in response to tryptophan deficiency.; Authors - Mingqing Cui
human,mouse	IHC	medRxiv; Secreted protein profiling of human aortic smooth muscle cells identifies vascular disease associations; Authors - Rédouane Aherrahrou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Udesh (Verified Customer) (10-05-2022)

The Ab was used at 1:1000 dilution in NFDM overnight 4 degrees and detected a band close to 220 kD in 20ug cell lysate. Also tested for for positive IF staining at 1:250 dilution in 2% BSA overnight at 4 degrees.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Bone Marrow MSCs

LTBP1 Antibody Western Blot validation (1:1000 dilution) in Bone Marrow MSCs (Cat no:26855-1-AP)