LTBP2 Polyklonaler Antikörper

LTBP2 Polyklonal Antikörper für IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 28824-1-AP

Synonyme

C14orf141, LTBP3, Latent-transforming growth factor beta-binding protein 2, LTBP 2, LTBP-2

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  IP experiment of U2OS using 28824-1-AP

  IP result of anti-LTBP2 (IP:28824-1-AP, 4ug; Detection:28824-1-AP 1:600) with U2OS cells lysate 1840 ug.

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  IHC staining of mouse lung using 28824-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28824-1-AP (LTBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 28824-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28824-1-AP (LTBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 28824-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 28824-1-AP (LTBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 28824-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using LTBP2 antibody (28824-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

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Geprüfte Anwendungen

• Erfolgreiche IP: U2OS-Zellen
• Erfolgreiche Detektion in IHC: Mauslungengewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

28824-1-AP bindet in IHC, IF/ICC, IP, ELISA LTBP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LTBP2 fusion protein Ag29737
• Vollständiger Name: latent transforming growth factor beta binding protein 2
• Berechnetes Molekulargewicht: 1821 aa, 195 kDa
• Beobachtetes Molekulargewicht: 260 kDa
• GenBank-Zugangsnummer: BC078659
• Gene symbol: LTBP2
• Gene ID (NCBI): 4053
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LTBP2, an extracellular glycoprotein mapping to chromosome 14q24, belongs to a family of latent TGF-β binding proteins (LTBPs) that regulates a well-known growth factor, TGF-β. LTBP2 is an elastic fiber-associating protein whose function in elastogenesis is not clear, as a DANCEbinding Protein. (PMID: 17581631 )It may play an integral structural role in elastic-fiber architectural organization and/or assembly. LTBP2 associates covalently with small latent TGF-beta complex via Repeat B and Repeat C. Defects in LTBP2 are the cause of primary congenital glaucoma type 3D (GLC3D). [MIM:613086] LTBP2 is a role in the structural stability of ciliary zonules, and growth and development of lens.(PMID: 20617341) LTBP2 null mutations in an autosomal recessive ocular syndrome with megalocornea, spherophakia, and secondary glaucoma (PMID: 20179738 ) . LTBP2 is a biomarker of heart failure. LTBP2 has a molecular weight of 260 kDa (PMID: 20878956), and even larger molecular weights of 290 and 310 kDa were detected in tissue extracts. (PMID: 8524260)

Publikationen

Species	Application	Title
human	IHC	J Clin Endocrinol Metab; High-Throughput Proteomics and Immunohistochemistry of Orbital Connective Tissue in Graves' Orbitopathy; Authors - Yuanping Hai