Lactotransferrin/LTF Rekombinanter Antikörper

Lactotransferrin/LTF Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240280E2

Kat-Nr. : 83409-5-RR

Synonyme

Lactoferrin, LTF, HLF2, Growth-inhibiting protein 12, GIG12

---

• 

  WB analysis of human saliva using 83409-5-RR

  human saliva were subjected to SDS PAGE followed by western blot with 83409-5-RR (Lactotransferrin/LTF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 83409-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83409-5-RR against Human Lactotransferrin/LTF were performed. The affinity constant is 0.896 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human saliva

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83409-5-RR bindet in WB, ELISA Lactotransferrin/LTF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: lactotransferrin
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 30 kDa, 51 kDa, 80 kDa
• GenBank-Zugangsnummer: NM_002343.6
• Gene symbol: LTF
• Gene ID (NCBI): 4057
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LTF(Lactotransferrin) is also named as GIG12, LF and belongs to the transferrin family. It is an iron binding transport protein which can bind two atoms of ferric iron in association with the binding of an anion, usually bicarbonate. LTF is also an important protein component of the innate immune system that is broadly distributed within the body fluids. It can contribute to the activation of both the innate and adaptive immune responses by promoting the recruitment of leukocytes and activation of dendritic cells(PMID:18453607). This protein has 2 isoforms produced by alternative promoter usage. The human LTF can be isolated two fragments, an N-terminal tryptic fragment having an MW of 30 kDa and a C-terminal tryptic fragment having an MW of 50 kDa by a mild tryptic digest(PMID:3790094).