Lumican Polyklonaler Antikörper

Lumican Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10677-1-AP

Synonyme

KSPG lumican, LDC, LUM, lumican, SLRR2D

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 10677-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10677-1-AP (Lumican antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10677-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10677-1-AP (Lumican antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10677-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10677-1-AP (Lumican antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IF Staining of human colon using 10677-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Lumican antibody (10677-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen
• Erfolgreiche Detektion in IF: humanes Kolongewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

10677-1-AP bindet in WB, IF, ELISA Lumican und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Lumican fusion protein Ag1106
• Vollständiger Name: lumican
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 65-90 kDa
• GenBank-Zugangsnummer: BC007038
• Gene symbol: LUM
• Gene ID (NCBI): 4060
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lumican (LUM), a member of the small leucine-rich proteoglycan (SLRP) family, is one of the major extracellular components in interstitial collagenous matrices of the corneal stroma, aorta, skin, skeletal muscle, lung, kidney, bone, cartilage, and intervertebral discs. SLRPs constitute an important fraction of noncollagenous extracellular matrix proteins and have important effects on cell behavior. Lumican regulates collagenous matrix assembly as a keratan sulfate proteoglycan in the cornea and may exist as a glycoprotein in the connective tissues of other organs. Posttranslational modifications lumican goes through can increase its apparent molecular weight to 50-90 kDa. Studies show that lumican participates in the maintenance of tissue homeostasis and modulates cellular functions including cell proliferation, migration, and differentiation. (PMID: 18949819; 22252641; 9761754; 22365843)

Publikationen

Species	Application	Title
human,rat	WB	Front Mol Biosci; Integrated Strategies of Diverse Feature Selection Methods Identify Aging-Based Reliable Gene Signatures for Ischemic Cardiomyopathy.; Authors - Huafeng Song
human	WB	J Proteomics; Protein expression profiles of human lymph and plasma mapped by 2D-DIGE and 1D SDS-PAGE coupled with nanoLC-ESI-MS/MS bottom-up proteomics.; Authors - Clement Cristina C CC
human	WB	J Cell Physiol; Priming integrin α5 promotes human dental pulp stem cells odontogenic differentiation due to extracellular matrix deposition and amplified extracellular matrix-receptor activity.; Authors - He Wang