LUZP1 Polyklonaler Antikörper

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• KD/KO Validated

LUZP1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 17483-1-AP

Synonyme

LUZP

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  WB analysis of mouse brain using 17483-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17483-1-AP (LUZP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17483-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17483-1-AP (LUZP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17483-1-AP

  IP result of anti-LUZP1 (IP:17483-1-AP, 4ug; Detection:17483-1-AP 1:10000) with mouse brain tissue lysate 1680 ug.

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  IHC staining of human ovary tumor using 17483-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17483-1-AP (LUZP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 17483-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17483-1-AP (LUZP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 17483-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17483-1-AP (LUZP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

17483-1-AP bindet in WB, IHC, IF, IP, ELISA LUZP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LUZP1 fusion protein Ag11518
• Vollständiger Name: leucine zipper protein 1
• Berechnetes Molekulargewicht: 1026 aa, 115 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC051733
• Gene symbol: LUZP1
• Gene ID (NCBI): 7798
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LUZP1, also known as LUZP, is predominantly expressed in the brain. LUZP1 is a centrosome, actin, and midbody-localizing protein implicated in ciliogenesis regulation and actin cytoskeleton stability (PMID: 33869174). LUZP1 null mice present defective neural tube closure and cardiovascular problems, which cause perinatal death. In addition, LUZP1 has also been implicated in the etiology of diseases like the 1p36 and the Townes-Brocks syndromes (PMID: 35738212).

Publikationen

Species	Application	Title
human	WB	Elife; LUZP1, a novel regulator of primary cilia and the actin cytoskeleton, is a contributing factor in Townes-Brocks Syndrome.; Authors - Laura Bozal-Basterra
human	WB	Front Oncol; The Circ_0001367/miR-545-3p/LUZP1 Axis Regulates Cell Proliferation, Migration and Invasion in Glioma Cells.; Authors - Xuchen Dong
mouse	WB	J Biol Chem; Activating transcription factor 4 (ATF4) promotes skeletal muscle atrophy by forming a heterodimer with the transcriptional regulator C/EBPβ.; Authors - Scott M Ebert
		Eur J Cell Biol; LUZP1: A new player in the actin-microtubule cross-talk.; Authors - João Gonçalves
mouse	WB	Biochem Cell Biol; Chromatin remodeling factor CECR2 forms tissue-specific complexes with CCAR2 and LUZP1.; Authors - Farshad Niri
human	WB	bioRxiv; Histone chaperone HIRA, Promyelocytic Leukemia (PML) protein and p62/SQSTM1 coordinate to regulate inflammation during cell senescence; Authors - Nirmalya Dasgupta