LXN Monoklonaler Antikörper

LXN Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen

Anwendung

WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3E5A5

Kat-Nr. : 66957-1-PBS

Synonyme

TCI, Protein MUM, Latexin, ECI, 3E5A5

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  WB analysis of pig brain using 66957-1-Ig (same clone as 66957-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66957-1-Ig (LXN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66957-1-Ig (same clone as 66957-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66957-1-Ig (LXN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66957-1-Ig (same clone as 66957-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66957-1-Ig (LXN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 66957-1-Ig (same clone as 66957-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66957-1-Ig (LXN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit brain using 66957-1-Ig (same clone as 66957-1-PBS)

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 66957-1-Ig (LXN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66957-1-Ig (same clone as 66957-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66957-1-Ig (LXN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP50023-1

  Sandwich ELISA standard curve ofMP50023-1, LXN Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66957-1-PBS. Detection antibody: HRP-conjugated 66957-2-PBS. Standard: Ag27922. Range: 0.313-20 ng/mL.

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  IHC staining of human heart using 66957-1-Ig (same clone as 66957-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66957-1-Ig (LXN antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66957-1-Ig (same clone as 66957-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66957-1-Ig (LXN antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66957-1-Ig (same clone as 66957-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using LXN antibody (66957-1-Ig, Clone: 3E5A5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66957-1-Ig (same clone as 66957-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human LXN (66957-1-Ig, Clone:3E5A5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66957-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66957-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA LXN und zeigt Reaktivität mit Hausschwein, human, Kaninchen

• Getestete Reaktivität: Hausschwein, human, Kaninchen
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LXN fusion protein Ag27922
• Vollständiger Name: latexin
• Berechnetes Molekulargewicht: 222 aa, 26 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC005346
• Gene symbol: LXN
• Gene ID (NCBI): 56925
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Latexin, also named as LTXN, LXN, ECI, MUM and TCI, is hardly reversible, non-competitive, and potent inhibitor of CPA1, CPA2 and CPA4. It may function as a neuroprotective mechanism against tau toxicity.