LYN Monoklonaler Antikörper

LYN Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3C7F2

Kat-Nr. : 60211-1-PBS

Synonyme

JTK8, LYN, Tyrosine protein kinase Lyn

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  WB analysis of Jurkat using 60211-1-Ig (same clone as 60211-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60211-1-Ig (LYN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60211-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60211-1-Ig (same clone as 60211-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 60211-1-Ig (LYN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60211-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 60211-1-Ig (same clone as 60211-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60211-1-Ig (LYN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60211-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 60211-1-Ig (same clone as 60211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60211-1-Ig (LYN Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60211-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 60211-1-Ig (same clone as 60211-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60211-1-Ig (LYN Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60211-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60211-1-Ig (same clone as 60211-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using LYN antibody (60211-1-Ig, Clone: 3C7F2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60211-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60211-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA LYN und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: LYN fusion protein Ag12723
• Vollständiger Name: v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
• Berechnetes Molekulargewicht: 512 aa, 59 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC075002
• Gene symbol: LYN
• Gene ID (NCBI): 4067
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.