LYPD3 Rekombinanter Antikörper

LYPD3 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

240347C5

Kat-Nr. : 83345-1-RR

Synonyme

MIG C4, LY6/PLAUR domain containing 3, C4.4A, 240347C5

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  WB analysis using 83345-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83345-1-RR (LYPD3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83345-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83345-1 -RR against Human LYPD3 were performed. The affinity constant is 3.04 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HaCaT-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

83345-1-RR bindet in WB, IHC, ELISA LYPD3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: LYPD3 fusion protein Ag34961
• Vollständiger Name: LY6/PLAUR domain containing 3
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 75-80 kDa
• GenBank-Zugangsnummer: NM_014400
• Gene symbol: LYPD3
• Gene ID (NCBI): 27076
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LYPD3 (Ly6/PLAUR domain-containing protein 3), also known as C4.4A , is a membrane protein anchored to the cell surface via a glycosylphosphatidylinositol (GPI) moiety (PMID: 25302166; 15012588). It is a tumorigenic and high-glycosylated protein that has been proven to be linked with the carcinogenic effects in different solid tumors, such as lung cancer and breast cancer (PMID: 32040344; 29371917; 31644911). It has been shown that the elevated expression of LYPD3 is associated with lung adenocarcinoma carcinogenesis and poor prognosis (PMID: 25302166; 32040344).

Publikationen

Species	Application	Title
human	IHC	J Cell Mol Med; Unveiling Varied Cell Death Patterns in Lung Adenocarcinoma Prognosis and Immunotherapy Based on Single-Cell Analysis and Machine Learning; Authors - Zipei Song