Lysozyme Polyklonaler Antikörper

Lysozyme Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Rezensionen (2)

Kat-Nr. : 15013-1-AP

Synonyme

1,4 beta N acetylmuramidase C, lysozyme, lysozyme (renal amyloidosis), Lysozyme C, LYZ, LZM

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Galerie der Validierungsdaten

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  WB analysis of human spleen using 15013-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 15013-1-AP (LYZ antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 15013-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 15013-1-AP (LYZ antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 15013-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 15013-1-AP (Lysozyme antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15013-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15013-1-AP (LYZ antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 15013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15013-1-AP (Lysozyme antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 15013-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15013-1-AP (Lysozyme antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: THP-1-Zellen, HL-60-Zellen, humanes Milzgewebe, Mauslungengewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IF: See 2 publications below

Produktinformation

15013-1-AP bindet in WB, IHC, IF, ELISA Lysozyme und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Lysozyme fusion protein Ag6917
• Vollständiger Name: lysozyme (renal amyloidosis)
• Berechnetes Molekulargewicht: 17 kDa
• Beobachtetes Molekulargewicht: 15-17 kDa
• GenBank-Zugangsnummer: BC004147
• Gene symbol: LYZ
• Gene ID (NCBI): 4069
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.

Publikationen

Species	Application	Title
		Cell Chem Biol; Engineered Glycocalyx Regulates Stem Cell Proliferation in Murine Crypt Organoids.; Authors - Sara H Rouhanifard
human		Arch Toxicol; Extending the viability of human precision-cut intestinal slice model for drug metabolism studies.; Authors - C Biel
mouse	WB	FASEB J; Loss of interleukin-10 receptor disrupts intestinal epithelial cell proliferation and skews differentiation towards the goblet cell fate.; Authors - Brittany R Jenkins
mouse		Int J Mol Sci; Nrf2-Knockout Protects from Intestinal Injuries in C57BL/6J Mice Following Abdominal Irradiation with γ Rays.; Authors - Wenyan Yang
rat	IF	Physiol Genomics; The Atg16l1 gene: characterization of wild type, knock-in, and knock-out phenotypes in rats.; Authors - Kari Chesney
human		Histol Histopathol; Immunohistochemical study of inflammatory responses in septa arising from type I intestinal atresia.; Authors - Xuelai Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Balawant (Verified Customer) (12-20-2022)

I have used this antibody for colon tissue and colon cell line. this antibody is working great and no background.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: colon tissue and colon cell line (caco2 cells)

FH

Claudio (Verified Customer) (12-15-2021)

The antibody staining did not yield any labelling, though it was tested in paraffin sections so you might get better results with cryostat sections.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse heart sections