LZTFL1 Polyklonaler Antikörper

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• KD/KO Validated

LZTFL1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 17073-1-AP

Synonyme

LZTFL1

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Galerie der Validierungsdaten

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  WB analysis using 17073-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17073-1-AP (LZTFL1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 17073-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17073-1-AP (LZTFL1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 17073-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17073-1-AP (LZTFL1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human small intestine using 17073-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17073-1-AP (LZTFL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 17073-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 17073-1-AP (LZTFL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 17073-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17073-1-AP (LZTFL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 17073-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17073-1-AP (LZTFL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 17073-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 17073-1-AP (LZTFL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 17073-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 17073-1-AP (LZTFL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17073-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17073-1-AP (LZTFL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat heart using 17073-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 17073-1-AP (LZTFL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 17073-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 17073-1-AP (LZTFL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 17073-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17073-1-AP (LZTFL1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 17073-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17073-1-AP (LZTFL1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 17073-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17073-1-AP (LZTFL1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 17073-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 17073-1-AP (LZTFL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Maushodengewebe, Rattenhodengewebe, U-251-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe, humanes Dünndarmgewebe, humanes Hodengewebe, humanes Kolongewebe, humanes Lungengewebe, Maus-Dünndarmgewebe, Mausherzgewebe, Maushodengewebe, Mauslungengewebe, Ratten-Dünndarmgewebe, Rattenherzgewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

17073-1-AP bindet in WB, IP, IHC, IF, ELISA LZTFL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LZTFL1 fusion protein Ag10919
• Vollständiger Name: leucine zipper transcription factor-like 1
• Berechnetes Molekulargewicht: 299 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC025988
• Gene symbol: LZTFL1
• Gene ID (NCBI): 54585
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LZTFL1 belongs to the LZTFL1 family. It is an important regulator of BBSome ciliary trafficking and hedgehog signaling. LZTFL1 regulates Ciliary Trafficking of the BBSome and Smoothened.(PMID:22072986)

Publikationen

Species	Application	Title
mouse	WB,IF	Dev Cell; IFT27 Links the BBSome to IFT for Maintenance of the Ciliary Signaling Compartment.; Authors - Thibaut Eguether ; KO Validated
mouse	WB,IF	EMBO J; Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.; Authors - Shichao Duan
mouse	IF	J Cell Biol; Ubiquitin links smoothened to intraflagellar transport to regulate Hedgehog signaling.; Authors - Paurav B Desai
human	WB,IHC,IP	Oncogene; LZTFL1 inhibits kidney tumor cell growth by destabilizing AKT through ZNRF1-mediated ubiquitin proteosome pathway; Authors - Jun Lu