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LZTFL1 Monoklonaler Antikörper

LZTFL1 Monoklonal Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2C1F6

Kat-Nr. : 68281-1-PBS

Synonyme

2C1F6, Leucine zipper transcription factor-like protein 1

Filter:
  • Western Blot (WB) analysis of various lysates using LZTFL1 Monoclonal antibody (68281-1-Ig)

    WB analysis using 68281-1-Ig (same clone as 68281-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68281-1-Ig (LZTFL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68281-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using LZTFL1 Monoclonal antibody (68281-1-Ig)

    IF Staining of Jurkat using 68281-1-Ig (same clone as 68281-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using LZTFL1 antibody (68281-1-Ig, Clone: 2C1F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68281-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using LZTFL1 Monoclonal antibody (68281-1-Ig)

    IF Staining of A431 using 68281-1-Ig (same clone as 68281-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using LZTFL1 antibody (68281-1-Ig, Clone: 2C1F6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 68281-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Jurkat cells using LZTFL1 Monoclonal antibody (68281-1-Ig)

    FC experiment of Jurkat using 68281-1-Ig (same clone as 68281-1-PBS)

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human LZTFL1 (68281-1-Ig, Clone:2C1F6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68281-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

68281-1-PBS bindet in WB, IF/ICC, FC (Intra), Indirect ELISA LZTFL1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen LZTFL1 fusion protein Ag10733
Vollständiger Name leucine zipper transcription factor-like 1
Berechnetes Molekulargewicht 299 aa, 35 kDa
Beobachtetes Molekulargewicht 35 kDa
GenBank-ZugangsnummerBC025988
Gene symbol LZTFL1
Gene ID (NCBI) 54585
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LZTFL1 belongs to the LZTFL1 family. It is an important regulator of BBSome ciliary trafficking and hedgehog signaling. LZTFL1 regulates Ciliary Trafficking of the BBSome and Smoothened.(PMID:22072986)