LZTS1 Polyklonaler Antikörper

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• KD/KO Validated

LZTS1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 20878-1-AP

Synonyme

Fez1, F37/esophageal cancer-related gene-coding leucine-zipper motif, F37

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  WB analysis using 20878-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 20878-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 20878-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 20878-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 20878-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20878-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 20878-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 20878-1-AP (LZTS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 20878-1-AP

  IP result of anti-LZTS1 (IP:20878-1-AP, 3ug; Detection:20878-1-AP 1:500) with mouse brain tissue lysate 7500ug.

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  IHC staining of human skin cancer using 20878-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 20878-1-AP (LZTS1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 20878-1-AP

  Immunofluorescent analysis of HepG2 cells, using LZTS1 antibody 20878-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, humanes Hodengewebe, Maushirngewebe, Maus-Eierstockgewebe, Maus-Thymusgewebe, PC-3-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

20878-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA LZTS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LZTS1 fusion protein Ag14977
• Vollständiger Name: leucine zipper, putative tumor suppressor 1
• Berechnetes Molekulargewicht: 596 aa, 67 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC075007
• Gene symbol: LZTS1
• Gene ID (NCBI): 11178
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IF	Nat Commun; Lzts1 controls both neuronal delamination and outer radial glial-like cell generation during mammalian cerebral development.; Authors - T Kawaue ; KO Validated
human	WB	Mol Oncol; Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis; Authors - Tianwei Chen
human	WB,IHC	Int Dent J; Screening Differentially Expressed Proteins in Areca Nut-Related Oral Squamous Cell Carcinoma Using Tandem Mass Tag Proteomics; Authors - Xiaoqun Mai