LZTS2 Polyklonaler Antikörper

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• KD/KO Validated

LZTS2 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 15677-1-AP

Synonyme

hLZTS2, KIAA1813, LAPSER1, LZTS2, Protein LAPSER1

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Galerie der Validierungsdaten

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  WB analysis using 15677-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15677-1-AP (LZTS2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15677-1-AP

  WB result of LZTS2 antibody (15677-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LZTS2 transfected HeLa cells.

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  IHC staining of human prostate hyperplasia using 15677-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15677-1-AP (LZTS2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate hyperplasia using 15677-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 15677-1-AP (LZTS2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 15677-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15677-1-AP (LZTS2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 15677-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15677-1-AP (LZTS2 Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatahyperplasie-Gewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Produktinformation

15677-1-AP bindet in WB, IP, IHC, IF, ELISA LZTS2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: LZTS2 fusion protein Ag8145
• Vollständiger Name: leucine zipper, putative tumor suppressor 2
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC006212
• Gene symbol: LZTS2
• Gene ID (NCBI): 84445
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

LZTS2, also named as KIAA1813 and LAPSER1, is a novel b-Catenin-interacting protein and it regulates the nuclear export of b-Catenin. LZTS2 is a negative regulator of katanin-mediated microtubule severing and release from the centrosome. It is required for central spindle formation and the completion of cytokinesis.

Publikationen

Species	Application	Title
	IF	Nat Commun; Proximity proteomics identifies PAK4 as a component of Afadin-Nectin junctions.; Authors - Yohendran Baskaran
human	WB,IP	Oncogene; β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.; Authors - Yanwei Lu ; KD Validated
human	WB	Cancer Lett; LZTS2 inhibits PI3K/AKT activation and radioresistance in nasopharyngeal carcinoma by interacting with p85.; Authors - Shuangbing Xu ; KD Validated
human	WB,IF	bioRxiv; α-catenin mechanosensitivity as a route to cytokinesis failure through sequestration of LZTS2; Authors - Yuou Wang ; KD Validated
human	WB	Cell Death Dis; RRP15 deficiency induces ribosome stress to inhibit colorectal cancer proliferation and metastasis via LZTS2-mediated β-catenin suppression; Authors - Zhixiong Dong
human	WB	Biochem Pharmacol; EBV-encoded miRNAs BHRF1-1 and BART2-5p aggravate post- transplant lymphoproliferative disorder via LZTS2-PI3K-AKT axis; Authors - Hao Ji