Lamin B1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Lamin B1 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12987-1-PBS

Synonyme

LMNB1, laminB1, Lamin-B1, LMN2, LMNB

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  WB analysis using 12987-1-AP (same clone as 12987-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12987-1-AP (same clone as 12987-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12987-1-AP (same clone as 12987-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12987-1-AP (Lamin B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 12987-1-AP (same clone as 12987-1-PBS)

  IP result of anti-Lamin B1 (IP:12987-1-AP, 4ug; Detection:12987-1-AP 1:50000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 12987-1-AP (same clone as 12987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 12987-1-AP (same clone as 12987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 12987-1-AP (same clone as 12987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 12987-1-AP (same clone as 12987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 12987-1-AP (same clone as 12987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 12987-1-AP (same clone as 12987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 12987-1-AP (same clone as 12987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12987-1-AP (Lamin B1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12987-1-AP (same clone as 12987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 12987-1-AP (LMNB1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 12987-1-AP (same clone as 12987-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 12987-1-AP (LMNB1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

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  IF Staining of human skin cancer using 12987-1-AP (same clone as 12987-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human skin cancer tissue using 12987-1-AP (Lamin B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12987-1-AP (same clone as 12987-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 12987-1-AP (Lamin B1 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12987-1-AP (same clone as 12987-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Lamin B1 antibody (12987-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12987-1-AP (same clone as 12987-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using LMNB1 antibody 12987-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 12987-1-AP (same clone as 12987-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 12987-1-AP (Lamin B1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 12987-1-AP (same clone as 12987-1-PBS)

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human Lamin B1 (12987-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12987-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12987-1-PBS bindet in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Indirect ELISA Lamin B1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Lamin B1 fusion protein Ag3631
• Vollständiger Name: lamin B1
• Berechnetes Molekulargewicht: 66 kDa
• Beobachtetes Molekulargewicht: 66-70 kDa
• GenBank-Zugangsnummer: BC012295
• Gene symbol: Lamin B1
• Gene ID (NCBI): 4001
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Expression of uncleavable mutant lamin A or B caused significant delays in the onset of chromatin condensation and nuclear shrinkage during apoptosis (PMID:11953316). This protein is not suitable for samples where the nuclear envelope has been removed.