Lamin B2 Rekombinanter Antikörper

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• KD/KO Validated

Lamin B2 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230184E7

Kat-Nr. : 82801-2-PBS

Synonyme

LMNB2, 230184E7, LAMB2, Lamin-B2, LMN2

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  WB analysis using 82801-2-RR (same clone as 82801-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82801-2-RR (Lamin B2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82801-2-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 82801-2-RR (same clone as 82801-2-PBS)

  WB result of Lamin B2 antibody (82801-2-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin B2 transfected A549 cells. This data was developed using the same antibody clone with 82801-2-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 82801-2-RR (same clone as 82801-2-PBS)

  Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 82801-2-RR (Lamin B2 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82801-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82801-2-RR (same clone as 82801-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Lamin B2 antibody (82801-2-RR, Clone: 230184E7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82801-2-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 82801-2-RR (same clone as 82801-2-PBS)

  (blue)1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human Lamin B2 (82801-2-RR, Clone:230184E7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166(red), or 0.25 ug rabbit IgG isotype control. Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82801-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82801-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82801-2-RR against Human Lamin B2 were performed. The affinity constant is 2.81 nM.

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Geprüfte Anwendungen

Produktinformation

82801-2-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Lamin B2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Lamin B2 fusion protein Ag1335
• Vollständiger Name: lamin B2
• Berechnetes Molekulargewicht: 68 kDa
• Beobachtetes Molekulargewicht: 68 kDa
• GenBank-Zugangsnummer: BC006551
• Gene symbol: Lamin B2
• Gene ID (NCBI): 84823
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lamins are nuclear membrane structural components that are important in structural integrity of the nucleus and may also interact with chromatin (PMID: 33033404). Research studies show that lamin B2 knockout mice exhibit neuronal developmental defects and that both proteins are essential for typical brain development (PMID: 20145110). Mutations in Lamin B2 can result in a susceptibility to developing acquired partial lipodystrophy, a rare disorder characterized by the progressive loss of subcutaneous fat in a bilaterally symmetrical fashion (PMID: 16826530).